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electroporation buffer (sf cell line solution)  (Lonza)


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    Structured Review

    Lonza electroporation buffer (sf cell line solution)
    Electroporation Buffer (Sf Cell Line Solution), supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sf+solution/electroporation+buffer/pm40465629-246-14-20
    Average 90 stars, based on 1 article reviews
    electroporation buffer (sf cell line solution) - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Global organelle profiling reveals subcellular localization and remodeling at proteome scale
    Article Snippet: Electroporation was carried out in Amaxa 96-well shuttle Nucleofector device (Lonza) using SF solution (Lonza) following the manufacturer’s instructions.

    Article Title: High-throughput identification of functional regulatory SNPs in systemic lupus erythematosus
    Article Snippet: Daudi cells were then washed with PBS twice and resuspended in SF solution (Lonza, catalog number V4XC-2032), 300k cells per 20 μl solution.

    Article Title: Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κB.
    Article Snippet: This 5 mL reaction was then added to 20 mL of SF solution (Lonza catalog no. V4XC-2032) containing 400,000 NALM-6 cells.

    Transfection:

    Article Title: Targeting SRSF2 mutations in leukemia with RKI-1447: A strategy to impair cellular division and nuclear structure
    Article Snippet: .. OCI-AML2 cells were transfected in SF solution (V4XC-2032, Lonza), 300,000 cells/rxn, using DN-100 program. ..

    Microscopy:

    Article Title: OCA7 is a melanosome membrane protein that defines pigmentation by regulating early stages of melanosome biogenesis.
    Article Snippet: .. For microscopy experiments, 0.3 × 106 cells were resuspended in 20 μl Lonza SF solution, mixed with 0.4 μg of each plasmid, transferred to 16-well nucleocuvette strips, and nucleofected using program DS-137 in a Lonza 4D nucleofector. .. After 10 min, cells were transferred to 35 mm glass bottom dishes with 2 ml 37 C KGM Gold media and grown 24 to 72 h. Nonidentified human neonatal primary epidermal melanocytes (Thermo Fisher Scientific; catalog no.: C0025C) were cultured in supplemented Medium 254 per manufacturer instructions and nucleofected as described for MNT1 cells. imMKCL cells were cultured as previously described (58, 62) and nucleofected as described for MNT1 cells except program EN-138 was used for nucleofection.

    Plasmid Preparation:

    Article Title: OCA7 is a melanosome membrane protein that defines pigmentation by regulating early stages of melanosome biogenesis.
    Article Snippet: .. For microscopy experiments, 0.3 × 106 cells were resuspended in 20 μl Lonza SF solution, mixed with 0.4 μg of each plasmid, transferred to 16-well nucleocuvette strips, and nucleofected using program DS-137 in a Lonza 4D nucleofector. .. After 10 min, cells were transferred to 35 mm glass bottom dishes with 2 ml 37 C KGM Gold media and grown 24 to 72 h. Nonidentified human neonatal primary epidermal melanocytes (Thermo Fisher Scientific; catalog no.: C0025C) were cultured in supplemented Medium 254 per manufacturer instructions and nucleofected as described for MNT1 cells. imMKCL cells were cultured as previously described (58, 62) and nucleofected as described for MNT1 cells except program EN-138 was used for nucleofection.

    Article Title: Reliable multiplex generation of pooled induced pluripotent stem cells
    Article Snippet: .. The SF cell line solution was prepared fresh by combining 18.2ul of SF supplement, 81.2ul SF solution and 7.5ug plasmid per nucleofection, Lonza V4XC-2024. ..

    In Vitro:

    Article Title: PROTOSPACEJAM: AN OPEN-SOURCE, CUSTOMIZABLE AND WEB-ACCESSIBLE DESIGN PLATFORM FOR CRISPR/CAS9 INSERTIONAL KNOCK-IN
    Article Snippet: .. Briefly, ribonucleoprotein (RNP) S. pyogenes Cas9/gRNA complexes were prepared in vitro, mixed with HDR donor templates, and electroporated into HEK-293T cells by nucleofection using SF solution (Lonza #V4SC-2096) and the CM-130 program. .. Five days post-nucleofection, the distribution of fluorescence signal in each target cell population was analyzed by analytical flow cytometry on a FACSymphony instrument (BD Biosciences).



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